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<channel>
	<title>SOP &#8211; Coastal Ecosystems Research Group</title>
	<atom:link href="https://www.coastal-ecosystems-research.org/category/sop/feed/" rel="self" type="application/rss+xml" />
	<link>https://www.coastal-ecosystems-research.org</link>
	<description>We investigate the ecology, development and ecosystem services provided by coastal habitats in order to provide an evidence base for their restoration and creation. We are currently focused on the &#039;blue&#039; carbon storage potential and biodiversity value of restored coastal habitats.</description>
	<lastBuildDate>Fri, 27 Sep 2024 14:50:15 +0000</lastBuildDate>
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<image>
	<url>https://www.coastal-ecosystems-research.org/wp-content/uploads/2024/10/cropped-microscope-favicon-32x32.png</url>
	<title>SOP &#8211; Coastal Ecosystems Research Group</title>
	<link>https://www.coastal-ecosystems-research.org</link>
	<width>32</width>
	<height>32</height>
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	<item>
		<title>015 Total Suspended Solids</title>
		<link>https://www.coastal-ecosystems-research.org/2024/09/27/015-total-suspended-solids/</link>
					<comments>https://www.coastal-ecosystems-research.org/2024/09/27/015-total-suspended-solids/#respond</comments>
		
		<dc:creator><![CDATA[Robert Sparkes]]></dc:creator>
		<pubDate>Fri, 27 Sep 2024 14:50:15 +0000</pubDate>
				<category><![CDATA[SOP]]></category>
		<guid isPermaLink="false">https://www.defrostingthefreezer.co.uk/?p=720</guid>

					<description><![CDATA[Filtration of water samples to remove and measure suspended solids.]]></description>
										<content:encoded><![CDATA[
<div class="wp-block-file"><a id="wp-block-file--media-59070064-cce3-42c0-a944-5e447a6cb1da" href="https://www.coastal-ecosystems-research.org/wp-content/uploads/2024/09/BC_015-Total-Suspended-Solids-v1.0.docx">BC_015 Total Suspended Solids v1.0</a><a href="https://www.coastal-ecosystems-research.org/wp-content/uploads/2024/09/BC_015-Total-Suspended-Solids-v1.0.docx" class="wp-block-file__button wp-element-button" download aria-describedby="wp-block-file--media-59070064-cce3-42c0-a944-5e447a6cb1da">Download</a></div>



<p>Filtration of water samples to remove and measure suspended solids.</p>



<p></p>
]]></content:encoded>
					
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			</item>
		<item>
		<title>014 Nutrient Extraction</title>
		<link>https://www.coastal-ecosystems-research.org/2024/09/27/014-nutrient-extraction/</link>
					<comments>https://www.coastal-ecosystems-research.org/2024/09/27/014-nutrient-extraction/#respond</comments>
		
		<dc:creator><![CDATA[Robert Sparkes]]></dc:creator>
		<pubDate>Fri, 27 Sep 2024 14:46:06 +0000</pubDate>
				<category><![CDATA[SOP]]></category>
		<guid isPermaLink="false">https://www.defrostingthefreezer.co.uk/?p=717</guid>

					<description><![CDATA[Extracting nutrients from sediment using de-ionised water.]]></description>
										<content:encoded><![CDATA[
<div class="wp-block-file"><a id="wp-block-file--media-8a0ab888-503d-457a-8d99-cfefc0b8245b" href="https://www.coastal-ecosystems-research.org/wp-content/uploads/2024/09/BC_014-Nutrient-extraction-v1.0.docx">BC_014 Nutrient extraction v1.0</a><a href="https://www.coastal-ecosystems-research.org/wp-content/uploads/2024/09/BC_014-Nutrient-extraction-v1.0.docx" class="wp-block-file__button wp-element-button" download aria-describedby="wp-block-file--media-8a0ab888-503d-457a-8d99-cfefc0b8245b">Download</a></div>



<p>Extracting nutrients from sediment using de-ionised water.</p>
]]></content:encoded>
					
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			</item>
		<item>
		<title>013 Dilute Acid Digestion</title>
		<link>https://www.coastal-ecosystems-research.org/2024/09/27/013-dilute-acid-digestion/</link>
					<comments>https://www.coastal-ecosystems-research.org/2024/09/27/013-dilute-acid-digestion/#respond</comments>
		
		<dc:creator><![CDATA[Robert Sparkes]]></dc:creator>
		<pubDate>Fri, 27 Sep 2024 14:44:52 +0000</pubDate>
				<category><![CDATA[SOP]]></category>
		<guid isPermaLink="false">https://www.defrostingthefreezer.co.uk/?p=714</guid>

					<description><![CDATA[Extracting elements from sediments using dilute nitric acid.]]></description>
										<content:encoded><![CDATA[
<div class="wp-block-file"><a id="wp-block-file--media-199f1aa0-5ab9-4f74-98d9-d2626155a402" href="https://www.coastal-ecosystems-research.org/wp-content/uploads/2024/09/BC_013-Dilute-acid-digest-v1.0.docx">BC_013 Dilute acid digest v1.0</a><a href="https://www.coastal-ecosystems-research.org/wp-content/uploads/2024/09/BC_013-Dilute-acid-digest-v1.0.docx" class="wp-block-file__button wp-element-button" download aria-describedby="wp-block-file--media-199f1aa0-5ab9-4f74-98d9-d2626155a402">Download</a></div>



<p>Extracting elements from sediments using dilute (0.43 M) nitric acid.</p>



<p></p>
]]></content:encoded>
					
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			</item>
		<item>
		<title>012 BHP Extraction and Separation</title>
		<link>https://www.coastal-ecosystems-research.org/2024/09/27/012-bhp-extraction-and-separation/</link>
					<comments>https://www.coastal-ecosystems-research.org/2024/09/27/012-bhp-extraction-and-separation/#respond</comments>
		
		<dc:creator><![CDATA[Robert Sparkes]]></dc:creator>
		<pubDate>Fri, 27 Sep 2024 14:41:28 +0000</pubDate>
				<category><![CDATA[SOP]]></category>
		<guid isPermaLink="false">https://www.defrostingthefreezer.co.uk/?p=711</guid>

					<description><![CDATA[Extration, separation, acetylation and preparation of BHPs.]]></description>
										<content:encoded><![CDATA[
<div class="wp-block-file"><a id="wp-block-file--media-903108a7-e9ec-4721-ae69-cb0c279351e2" href="https://www.coastal-ecosystems-research.org/wp-content/uploads/2024/09/BC_012-BHP-Extraction-and-Separation.docx">BC_012 BHP Extraction and Separation</a><a href="https://www.coastal-ecosystems-research.org/wp-content/uploads/2024/09/BC_012-BHP-Extraction-and-Separation.docx" class="wp-block-file__button wp-element-button" download aria-describedby="wp-block-file--media-903108a7-e9ec-4721-ae69-cb0c279351e2">Download</a></div>



<p>Extracting lipids from dried sediments. Separating bacteriohopanepolyol (BHP) molecules from the total lipid extract. Acetylating BHP molecules and preparation for LC-MS analysis.</p>



<p>Citation: <a href="https://www.defrostingthefreezer.co.uk/characterization-of-diverse-bacteriohopanepolyols-in-a-permanently-stratified-hyper-euxinic-lake/" data-type="link" data-id="https://www.defrostingthefreezer.co.uk/characterization-of-diverse-bacteriohopanepolyols-in-a-permanently-stratified-hyper-euxinic-lake/">O&#8217;Beirne et al., 2022</a>.</p>



<p></p>
]]></content:encoded>
					
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			</item>
		<item>
		<title>011 Dry Bulk Density</title>
		<link>https://www.coastal-ecosystems-research.org/2024/09/27/011-dry-bulk-density/</link>
					<comments>https://www.coastal-ecosystems-research.org/2024/09/27/011-dry-bulk-density/#respond</comments>
		
		<dc:creator><![CDATA[Robert Sparkes]]></dc:creator>
		<pubDate>Fri, 27 Sep 2024 14:38:08 +0000</pubDate>
				<category><![CDATA[SOP]]></category>
		<guid isPermaLink="false">https://www.defrostingthefreezer.co.uk/?p=708</guid>

					<description><![CDATA[Sampling and measurement of dry bulk density.]]></description>
										<content:encoded><![CDATA[
<div class="wp-block-file"><a id="wp-block-file--media-258409df-5aad-4c03-9733-9eb7bba063a2" href="https://www.coastal-ecosystems-research.org/wp-content/uploads/2024/09/BC_011-Dry-Bulk-Density-v1.0.docx">BC_011 Dry Bulk Density v1.0</a><a href="https://www.coastal-ecosystems-research.org/wp-content/uploads/2024/09/BC_011-Dry-Bulk-Density-v1.0.docx" class="wp-block-file__button wp-element-button" download aria-describedby="wp-block-file--media-258409df-5aad-4c03-9733-9eb7bba063a2">Download</a></div>



<p>Sampling and measurement of the dry bulk density of surface sediments.</p>



<p></p>
]]></content:encoded>
					
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			</item>
		<item>
		<title>010 Sediment Core Sampling</title>
		<link>https://www.coastal-ecosystems-research.org/2024/09/27/010-sediment-core-sampling/</link>
					<comments>https://www.coastal-ecosystems-research.org/2024/09/27/010-sediment-core-sampling/#respond</comments>
		
		<dc:creator><![CDATA[Robert Sparkes]]></dc:creator>
		<pubDate>Fri, 27 Sep 2024 14:36:07 +0000</pubDate>
				<category><![CDATA[SOP]]></category>
		<guid isPermaLink="false">https://www.defrostingthefreezer.co.uk/?p=704</guid>

					<description><![CDATA[Collection of 1 metre sediment cores.]]></description>
										<content:encoded><![CDATA[
<div class="wp-block-file"><a id="wp-block-file--media-74f008b6-4db3-4dfd-8feb-0b44fa917028" href="https://www.coastal-ecosystems-research.org/wp-content/uploads/2024/09/BC_010-Sediment-Core-sampling-v1.0.docx">BC_010 Sediment Core sampling v1.0</a><a href="https://www.coastal-ecosystems-research.org/wp-content/uploads/2024/09/BC_010-Sediment-Core-sampling-v1.0.docx" class="wp-block-file__button wp-element-button" download aria-describedby="wp-block-file--media-74f008b6-4db3-4dfd-8feb-0b44fa917028">Download</a></div>



<p>Collection of 1 metre sediment cores using a steel auger.</p>



<p></p>
]]></content:encoded>
					
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			</item>
		<item>
		<title>009 Sediment Surface Sampling</title>
		<link>https://www.coastal-ecosystems-research.org/2024/09/27/009-sediment-surface-sampling/</link>
					<comments>https://www.coastal-ecosystems-research.org/2024/09/27/009-sediment-surface-sampling/#respond</comments>
		
		<dc:creator><![CDATA[Robert Sparkes]]></dc:creator>
		<pubDate>Fri, 27 Sep 2024 14:34:19 +0000</pubDate>
				<category><![CDATA[SOP]]></category>
		<guid isPermaLink="false">https://www.defrostingthefreezer.co.uk/?p=701</guid>

					<description><![CDATA[Collection of the top 15 cm of sediment.]]></description>
										<content:encoded><![CDATA[
<div class="wp-block-file"><a id="wp-block-file--media-ab69ed66-634d-4c9e-a7b8-7ba87bcbf004" href="https://www.coastal-ecosystems-research.org/wp-content/uploads/2024/09/BC_009-Surface-sampling-v1.0.docx">BC_009 Surface sampling v1.0</a><a href="https://www.coastal-ecosystems-research.org/wp-content/uploads/2024/09/BC_009-Surface-sampling-v1.0.docx" class="wp-block-file__button wp-element-button" download aria-describedby="wp-block-file--media-ab69ed66-634d-4c9e-a7b8-7ba87bcbf004">Download</a></div>



<p>Collection of the top 15 cm of sediment using a steel auger.</p>



<p></p>
]]></content:encoded>
					
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			</item>
		<item>
		<title>008 Preparing Elemental Analysis Foils</title>
		<link>https://www.coastal-ecosystems-research.org/2024/09/27/008-preparing-elemental-analysis-foils/</link>
					<comments>https://www.coastal-ecosystems-research.org/2024/09/27/008-preparing-elemental-analysis-foils/#respond</comments>
		
		<dc:creator><![CDATA[Robert Sparkes]]></dc:creator>
		<pubDate>Fri, 27 Sep 2024 14:32:45 +0000</pubDate>
				<category><![CDATA[SOP]]></category>
		<guid isPermaLink="false">https://www.defrostingthefreezer.co.uk/?p=698</guid>

					<description><![CDATA[Adding sediment to tin foil cups.]]></description>
										<content:encoded><![CDATA[
<div class="wp-block-file"><a id="wp-block-file--media-cad9fd39-533c-4d63-a88a-53903002421a" href="https://www.coastal-ecosystems-research.org/wp-content/uploads/2024/09/BC_008-Preparing-Elemental-Analysis-foils-v1.0.docx">BC_008 Preparing Elemental Analysis foils v1.0</a><a href="https://www.coastal-ecosystems-research.org/wp-content/uploads/2024/09/BC_008-Preparing-Elemental-Analysis-foils-v1.0.docx" class="wp-block-file__button wp-element-button" download aria-describedby="wp-block-file--media-cad9fd39-533c-4d63-a88a-53903002421a">Download</a></div>



<p>Adding sediment to tin foil cups prior to quantification of C, N, O, H or S via Elemental Analysis.</p>



<p></p>
]]></content:encoded>
					
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			</item>
		<item>
		<title>007 Furnacing Organic Matter</title>
		<link>https://www.coastal-ecosystems-research.org/2024/09/27/007-furnacing-organic-matter/</link>
					<comments>https://www.coastal-ecosystems-research.org/2024/09/27/007-furnacing-organic-matter/#respond</comments>
		
		<dc:creator><![CDATA[Robert Sparkes]]></dc:creator>
		<pubDate>Fri, 27 Sep 2024 14:30:37 +0000</pubDate>
				<category><![CDATA[SOP]]></category>
		<guid isPermaLink="false">https://www.defrostingthefreezer.co.uk/?p=695</guid>

					<description><![CDATA[Removal of organic matter from sediments using a furnace.]]></description>
										<content:encoded><![CDATA[
<div class="wp-block-file"><a id="wp-block-file--media-33f1a011-d4ab-40ec-825c-47aa787a1c11" href="https://www.coastal-ecosystems-research.org/wp-content/uploads/2024/09/BC_007-Furnacing-organic-matter-v1.2.docx">BC_007 Furnacing organic matter v1.2</a><a href="https://www.coastal-ecosystems-research.org/wp-content/uploads/2024/09/BC_007-Furnacing-organic-matter-v1.2.docx" class="wp-block-file__button wp-element-button" download aria-describedby="wp-block-file--media-33f1a011-d4ab-40ec-825c-47aa787a1c11">Download</a></div>



<p>Removal of organic matter from sediments using a moderate-temperature furnace.</p>
]]></content:encoded>
					
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			</item>
		<item>
		<title>006 Acid Decarbonation</title>
		<link>https://www.coastal-ecosystems-research.org/2024/09/27/006-acid-decarbonation/</link>
					<comments>https://www.coastal-ecosystems-research.org/2024/09/27/006-acid-decarbonation/#respond</comments>
		
		<dc:creator><![CDATA[Robert Sparkes]]></dc:creator>
		<pubDate>Fri, 27 Sep 2024 14:28:21 +0000</pubDate>
				<category><![CDATA[SOP]]></category>
		<guid isPermaLink="false">https://www.defrostingthefreezer.co.uk/?p=690</guid>

					<description><![CDATA[Removal of carbonate minerals using hydrochloric acid.]]></description>
										<content:encoded><![CDATA[
<div class="wp-block-file"><a id="wp-block-file--media-3060695e-5b0d-491d-9306-6a920efedbac" href="https://www.coastal-ecosystems-research.org/wp-content/uploads/2024/09/BC_006-Acid-decarbonation-v1.0.docx">BC_006 Acid decarbonation v1.0</a><a href="https://www.coastal-ecosystems-research.org/wp-content/uploads/2024/09/BC_006-Acid-decarbonation-v1.0.docx" class="wp-block-file__button wp-element-button" download aria-describedby="wp-block-file--media-3060695e-5b0d-491d-9306-6a920efedbac">Download</a></div>



<p>Removal of carbonate minerals (inorganic carbon) using hydrochloric acid.</p>



<p>Note: this process also removes some organic matter (publication in review).</p>



<p></p>
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